《植物生理学报》 2009, 45(11): 1070-1074

杨树叶中Na⁺ 的积累与其耐盐能力的关系

杨迎霞¹, 郏艳红², 聂莉莉², 张越², 陈受宜³, 王景安^1,*, 刘仲齐^2,*

1 天津师范大学化学与生命科学学院, 天津300384; 2 天津市农业生物技术研究中心, 天津300192; 3 中国科学院遗传与发育生物学研究所, 北京100101

通信作者：王景安；E-mail: zqliu733@yahoo.com.cn, jinganwang899@126.com；Tel: 022-3733857

摘　要：

以受体杨树品种‘107 号’ 和转基因杨树‘18-1’的一年生枝条为材料, 采用Hoagland营养液水培方法, 检测二者植株生 长以及根、茎、叶中 Na+ 和 K+ 含量变化的结果表明, 在含 100 mmol•L-1 NaCl 的 Hoagland 营养液中, 二者的生长速度都受到 明显抑制, 但后者的受抑程度较小; 叶中 Na+ 含量呈持续增加趋势, 9 d 后其叶中的 Na+ 含量显著高于前者。在二者叶片枯 萎程度相同的情况下, ‘18-1’ 叶中的 Na+ 含量是 ‘107 号 ’ 的 1.6 倍左右。在 4 mol•L-1 NaCl 溶液中, 它们的表皮细胞死亡率分 别为 42% 和 97%。说明后者比前者有更高的耐盐能力。

关键词：杨树; 耐盐; NTHK1; Na+; K+

收稿：2009-08-12   修定：2009-10-21

资助：国家重点基础研究发展计划(2006CB100102)和“ 十一五” 国家科技支撑计划(2007BAD67B02)。

Relationship between Accumulation of Na⁺ in Leaves and Salt Tolerance of Different Populus Genotypes

YANG Ying-Xia¹, JIA Yan-Hong², NIE Li-Li², ZHANG Yue², CHEN Shou-Yi³, WANG Jing-An^1,*, LIU Zhong-Qi^2,*

¹College of Chemistry and Biology, Tianjin Normal University, Tianjin 300384, China; ²Tianjin Research Center of Agricultural Biotechnology, Tianjin 300192, China; ³Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: WANG Jing-An; E-mail: zqliu733@yahoo.com.cn, jinganwang899@126.com; Tel: 022-3733857

Abstract:

Plant growth and changes of Na⁺ and K⁺ concentrations in leaves, stems or roots were investigated in 1-year-old cuttings from receptor poplar ‘107’ (Populus×euramericana ‘Neva’) and transgenic poplar ‘18-1’, which were exposed to salt stress in Hoagland solution. It was shown that the growth of both poplars were markedly inhibited in Hoagland with 100 mmol·L^-1 NaCl addition, while ‘18-1’ was less inhibited than ‘107’. The concentration of Na⁺ in leaves was keeping increasing, and after 9 day culture Na⁺ concentration in the leaves of‘18-1’ was greatly higher than that of ‘107’. The accumulation amount of Na⁺ in ‘18-1’ leaves was about 1.6 times higher than that in ‘107’ when their leaves displayed same necrosis degree under NaCl stress. The death ratio of epidermal cells was about 42% and 97% in ‘18-1’ and ‘107’ in distilled water with 4 mol·L^-1 NaCl addition, respectively. These results indicated that salt tolerance of the transgenic poplar ‘18-1’ was much higher than that of receptor poplar ‘107’.

Key words: poplar; salt tolerance; NTHK1; Na⁺; K⁺